A Study of Saccharomyces cerevisiae Cell Wall Glucans

作者：Kwiatkowski Stefan^*; Thielen Ursula; Glenney Phyllis; Moran Colm

来源：Journal of the Institute of Brewing, 2009, 115(2): 151-158.

DOI：10.1002/j.2050-0416.2009.tb00361.x

• (1 -> 4)-alpha-D-glucan, 1 -> 6)-beta-D-glucan
• glucan specific antibodies
• NMR
• Saccharomyces cerevisiae
• yeast cell wall

Much research has been carried out over the years examining cell wall glucans from Saccharomyces cerevisiae and this Study further examines aspects of the binding of (1-->4)-alpha-D-glucan in the yeast cell wall, using a number of isolation techniques as well as monoclonal antibodies able to recognize a mixed (1-->4)-alpha-D-glucan/(1-->6)-beta-D-glucan. Extraction of purified glucan, from S. cerevisiae cell wall, with 0.1N HCl, at 80 degrees C for 6 h., released into the solution (1-->4)-alpha-D-glucan and (1-->6)-beta-D-glucan as the major polysaccharides, along with an insoluble pellet highly enriched in (1-->3)-beta-D-glucan. The released (1-->4)-alpha-D-glucan was composed of a high molecular size >100 kDa fraction (7.2% w/w) and a medium 5-50 kDa polysaccharide (10.2% w/w), with the (1-->4)-alpha-D-glucan covalently bound to the (1-->6)-beta-D-glucan. The average molar ratio of the alpha:beta glucan was 47: 53 in this mixed polysaccharide. The structure of this polysaccharide was different from the structure of plant starch or animal glycogen as monoclonal antibodies specific to yeast (1-->4)-alpha-D-glucan/(1-->6)-beta-D-glucan did not recognize the plant starch or animal glycogen standards.

• 出版日期2009