Acinetobacter species in the skin microbiota protect against allergic sensitization and inflammation

作者：Fyhrquist Nanna; Ruokolainen Lasse; Suomalainen Alina; Lehtimaki Sari; Veckman Ville; Vendelin Johanna; Karisola Piia; Lehto Maili; Savinko Terhi; Jarva Hanna; Kosunen Timo U; Corander Jukka; Auvinen Petri; Paulin Lars; von Hertzen Leena; Laatikainen Tiina; Makela Mika; Haahtela Tari; Greco Dario; Hanski Ilkka; Alenius Harri^*

来源：Journal of Allergy and Clinical Immunology, 2014, 134(6): 1301-+.

DOI：10.1016/j.jaci.2014.07.059

• Atopy
• Gammaproteobacteria
• Acinetobacter species
• PBMC
• anti-inflammatory gene expression
• dendritic cells
• keratinocytes
• mouse asthma model

Background: The human commensal microbiota interacts in a complex manner with the immune system, and the outcome of these interactions might depend on the immune status of the subject. %26lt;br%26gt;Objective: Previous studies have suggested a strong allergy-protective effect for Gammaproteobacteria. Here we analyze the skin microbiota, allergic sensitization (atopy), and immune function in a cohort of adolescents, as well as the influence of Acinetobacter species on immune responses in vitro and in vivo. %26lt;br%26gt;Methods: The skin microbiota of the study subjects was identified by using 16S rRNA sequencing. PBMCs were analyzed for baseline and allergen-stimulated mRNA expression. In in vitro assays human monocyte-derived dendritic cells and primary keratinocytes were incubated with Acinetobacter lwoffii. Finally, in in vivo experiments mice were injected intradermally with A lwoffii during the sensitization phase of the asthma protocol, followed by readout of inflammatory parameters. %26lt;br%26gt;Results: In healthy subjects, but not in atopic ones, the relative abundance of Acinetobacter species was associated with the expression of anti-inflammatory molecules by PBMCs. %26lt;br%26gt;Moreover, healthy subjects exhibited a robust balance between anti-inflammatory and T(H)1/T(H)2 gene expression, which was related to the composition of the skin microbiota. In cell assays and in a mouse model, Acinetobacter species induced strong T(H)1 and anti-inflammatory responses by immune cells and skin cells and protected against allergic sensitization and lung inflammation through the skin. %26lt;br%26gt;Conclusion: These results support the hypothesis that skin commensals play an important role in tuning the balance of T(H)1, T(H)2, and anti-inflammatory responses to environmental allergens.

• 出版日期2014-12