Analysis of the Genetic Diversity and Relationship within Hypsizygus marmoreus Based on Sequence - Related Amplification Polymorphism

作者：Qiu Chengshu; Yan Wenjuan; Song Bin; Li Peng; Deng Wangqiu; Li Taihui^*

来源：Journal of Pure and Applied Microbiology, 2013, 7(1): 1-9.

• Germplasm source
• evaluate
• Unweighted Pair Group Method with Arithmetic Mean (UPGMA)
• China

Germplasm resources of Hypsizygus marmoreus were collected from different parts of China. Sequence-related amplified polymorphism (SRAP) markers were employed to evaluate the genetic diversity and relationship among the 32 collected strains of H. marmoreus. Fifty-five SRAP primer combinations were used and 533 polymorphic bands were observed. Phylogenetic analysis by Unweighted Pair Group Method with Arithmetic Mean based on similarity matrices indicated three clusters, namely, A, B, and C. The Jaccard's similarity coefficients of genetic similarity among all accessions ranged from 0.40 to 0.73, and averaged 0.50 among these strains collected in China, revealing a medium level of genetic diversity. The results demonstrated that SRAP can be used to evaluate the genetic diversity of H. marmoreus in China and to distinguish the different storage and cultivar strains. These applications are helpful for the effective evaluation, management, utilization, and conservation of germplasm resources.

• 出版日期2013-3
• 单位华南理工大学