Italian ryegrass (Lolium multiflorum Lam) as a High-Potential Bio-Ethanol Resource

作者：Yasuda Masahide^*; Takenouchi Yuka; Nitta Yui; Ishii Yasuyuki; Ohta Kazuyoshi

来源：Bioenergy Research, 2015, 8(3): 1303-1309.

DOI：10.1007/s12155-015-9582-5

• Bio-ethanol
• Lignocellulosic raw material
• Italian ryegrass
• Simultaneous saccharification and co-fermentation (SSCF)

Cellulosic bioethanol production from lignocellulosic biomass has been recognized as one of the promising sustainable energy source alternatives to petroleum-based fuels, since the lignocelluloses are not directly in competition with food sources. In order to develop a lignocellulosic raw material with high energy crops for bio-ethanol production, we selected Italian ryegrass (Lolium multiflorum Lam). Bio-ethanol production from Italian ryegrass through a low-moisture anhydrous ammonia (LMAA) pretreatment and simultaneous saccharification and co-fermentation (SSCF) was examined. At first, Italian ryegrass was subjected to LMAA pretreatment, which was performed by keeping the moist powdered Italian ryegrass under NH3 gas atmosphere at room temperature for 4 weeks. The effect of LMAA pretreatment was examined for bio-ethanol production through simultaneous saccharification and fermentation (SSF) which was performed at 34 A degrees C using Saccharomyces cerevisiae, cellulase, and xylanase. The yields of ethanol and xylose in SSF were enhanced by LMAA-pretreatment. The SSCF of the LMAA-retreated Italian ryegrass was performed at 36 A degrees C using Escherichia coli KO11, S. cerevisiae, cellulase, and xylanase. The SSCF of LMAA-pretreated Italian ryegrass produced ethanol in higher yield (84.6 %) compared with those of LMAA-pretreated bamboo, rice straw, Napier grass, and silvergrass.

• 出版日期2015-9