Assessment of cardiac proteome dynamics with heavy water: slower protein synthesis rates in interfibrillar than subsarcolemmal mitochondria

作者：Kasumov Takhar^*; Dabkowski Erinne R; Shekar Kadambari Chandra; Li Ling; Ribeiro Rogerio F Jr; Walsh Kenneth; Previs Stephen F; Sadygov Rovshan G; Willard Belinda; Stanley William C

来源：American Journal of Physiology - Heart and Circulatory Physiology, 2013, 304(9): H1201-H1214.

DOI：10.1152/ajpheart.00933.2012

• deuterium
• heavy water
• heart
• high resolution mass spectroscopy
• proteome dynamics
• proteomics

Traditional proteomics provides static assessment of protein content, but not synthetic rates. Recently, proteome dynamics with heavy water ((H2O)-H-2) was introduced, where H-2 labels amino acids that are incorporated into proteins, and the synthesis rate of individual proteins is calculated using mass isotopomer distribution analysis. We refine this approach with a novel algorithm and rigorous selection criteria that improve the accuracy and precision of the calculation of synthesis rates and use it to measure protein kinetics in spatially distinct cardiac mitochondrial subpopulations. Subsarcolemmal mitochondria (SSM) and interfibrillar mitochondria (IFM) were isolated from adult rats, which were given (H2O)-H-2 in the drinking water for up to 60 days. Plasma (H2O)-H-2 and myocardial H-2-enrichment of amino acids were stable throughout the experimental protocol. Multiple tryptic peptides were identified from 28 proteins in both SSM and IFM and showed a time-dependent increase in heavy mass isotopomers that was consistent within a given protein. Mitochondrial protein synthesis was relatively slow (average half-life of 30 days, 2.4% per day). Although the synthesis rates for individual proteins were correlated between IFM and SSM (R-2 = 0.84; P %26lt; 0.0001), values in IFM were 15% less than SSM (P %26lt; 0.001). In conclusion, administration of (H2O)-H-2 results in stable enrichment of the cardiac precursor amino acid pool, with the use of refined analytical and computational methods coupled with cell fractionation one can measure synthesis rates for cardiac proteins in subcellular compartments in vivo, and protein synthesis is slower in mitochondria located among the myofibrils than in the subsarcolemmal region.

• 出版日期2013-5