A Novel Phage-Library-Selected Peptide Inhibits Human TNF-alpha Binding to Its Receptors

作者：Brunetti Jlenia; Lelli Barbara; Scali Silvia; Falciani Chiara; Bracci Luisa; Pini Alessandro^*

来源：Molecules, 2014, 19(6): 7255-7268.

DOI：10.3390/molecules19067255

• TNF-alpha
• anti-TNF-alpha peptide
• branched peptides
• phage display
• solid-phase synthesis
• competitive selection
• surface plasmon resonance

We report the identification of a new human tumor necrosis factor-alpha (TNF-alpha) specific peptide selected by competitive panning of a phage library. Competitive elution of phages was obtained using the monoclonal antibody adalimumab, which neutralizes pro- inflammatory processes caused by over-production of TNF-alpha in vivo, and is used to treat severe symptoms of rheumatoid arthritis. The selected peptide was synthesized in monomeric and branched form and analyzed for binding to TNF-alpha and competition with adalimumab and TNF-alpha receptors. Results of competition with TNF-alpha receptors in surface plasmon resonance and melanoma cells expressing both TNF receptors make the peptide a candidate compound for the development of a novel anti-TNF-alpha drug.

• 出版日期2014-6