Analysis of Flavonoids in Flos Genkwa by beta-cyclodextrin and Ionic Liquid Modified Capillary Zone Electrophoresis

作者：Lu Yuanqi; Li Yumei; Li Huixin^*

来源：Journal of the Chemical Society of Pakistan, 2008, 30(6): 829-835.

A beta-cyclodextrin (8-CD) and ionic liquid (1-ethyl-3-methylimidazolium tetrafluroborate, 1E-3MI-TFB) modified capillary zone electrophoretic (CZE) method has been developed to determine three flavonoids (genkwanin, apigenin and luteolin) in Flos Genkwa with 4-methylumbelliferone as internal standard. The effects of buffer pH and concentration, 8-CD concentration and ionic liquid on separation were investigated. The optimum electrophoretic conditions are as follows: 20 mmol . L(-1) tetraboratc (pH 9.0). 6 mmol . L(-1) beta-cyclodextrin-0.04% 1E-3MI-TFB (VIP) as the running buffer, applied voltage of 25 kV Under the optimized conditions, the calibration curves of the three flavonoids all showed excellent linearity in the 1 similar to 100 mu g . mL(-1) range. The relative standard deviation in migration time and peak area was 0.21% and 2.92% for genkwanin, 0.15% and 2.33% for apigenin, 0.28% and 3.35% for luteolin. The detection limits of the three flavonoids ranged from 0.26 to 0.32 mu g . mL(-1). The recoveries of the three flavonoids ranged between 94.3% and 103.5%.

• 出版日期2008-12
• 单位德州学院; 南京农业大学