Genetic Modification of the Marine-Derived Yeast Yarrowia lipolytica with High-Protein Content Using a GPI-Anchor-Fusion Expression System

作者：Wang Fang; Yue Lixi; Wang Lin; Li Jing; Wang Xianghong; Chi Zhenming^*; Madzak Catherine

来源：Biotechnology Progress, 2009, 25(5): 1297-1303.

DOI：10.1002/btpr.235

• single cell protein
• marine yeasts
• Y. lipolytica SWJ-1b
• surface display
• genetic modification

Yarrowia lipolytica SWJ-1b isolated from the marine fish gut was found to contain 47.6 g of crude protein per 100 g of cell dry weight and had potential use as single cell protein. When the gene encoding enhanced green fluorescent protein (EGFP) was inserted into the surface display plasmid pINA1317-YlCWP110 and expressed in uracil mutant Of Y. lipolytica SWJ-Ib, the corresponding protein was successfully displayed on the cell surface, and 100% of the yeast cells exhibited the anchored target proteins. We found that yeast cells displaying EGFP were similar to those of Y. lipolytica SWJ-1b. Furthermore, C(18:1) and C(18:3) fatty acids biosynthesis in the marine yeast cells displaying the heterologous EGFP was weakly enhanced compared with that in its wild-type. The results suggest that the marine-derived Y. lipolytica SWJ-1b can be armed with the heterologous protein by the genetic modification and further used as single cell protein.

• 出版日期2009-10
• 单位中国海洋大学