An improved method for quantitative analysis of total fructans in plant tissues

作者：Liu Zhiqian; Mouradov Aidyn; Smith Kevin F; Spangenberg German^*

来源：Analytical Biochemistry, 2011, 418(2): 253-259.

DOI：10.1016/j.ab.2011.08.004

• Fructan
• Quantitative analysis
• HPLC
• Perennial ryegrass

Current methods for measuring fructan levels in plant tissues are time-consuming and costly. They often involve multiple or sequential extractions, enzymatic or acid hydrolysis of fructan polymers, and multiple HPLC runs to quantify fructan-derived hexoses. Here we describe a new method that requires a single extraction step, followed by selective precipitation of fructans by acetone, acid hydrolysis of the precipitate, and a short (10 min) HPLC run to complete the procedure. We used perennial ryegrass samples to show that the new method has similar sensitivity, but better reproducibility, than a more complex method that is widely used. We have used the new method to study developmentally related changes in fructan levels in glasshouse-grown perennial ryegrass plants.

• 出版日期2011-11-15