An Amperometric Biosensor Based on Direct Immobilization of Horseradish Peroxidase on Electrochemically Reduced Graphene Oxide Modified Screen Printed Carbon Electrode

作者：Palanisamy Selvakumar^*; Unnikrishnan Binesh; Chen Shen Ming

来源：International Journal of Electrochemical Science, 2012, 7(9): 7935-7947.

• Horseradish peroxidase
• H2O2
• graphene oxide
• reduced graphene oxide
• amperometry
• immobilization

In this work we report the synthesis of graphene oxide - horseradish peroxidase biocomposite (GO-HRP) by a solution method without using any cross-linkers. Then, the GO-HRP biocomposite was drop casted on a screen printed carbon electrode (SPCE) followed by the electrochemical reduction to reduced graphene oxide-HRP (ERGO-HRP) composite. The ERGO-HRP modified SPCE showed good electroanalytical properties towards the amperometric determination of H2O2. The film has been characterized by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). Both the studies revealed the effective immobilization of HRP on ERGO. The SPCE/ERGO-HRP showed a linear range of detection of 9 - 195 mu M. The electrode also detects H2O2 in real sample solutions with appreciable accuracy.

• 出版日期2012-9