Development of amperometric L-tyrosine sensor based on Fe-doped hydroxyapatite nanoparticles

作者：Kanchana P; Lavanya N; Sekar C^*

来源：Materials Science and Engineering: C , 2014, 35: 85-91.

DOI：10.1016/j.msec.2013.10.013

• L-tyrosine
• tyrosinase
• Fe-doped hydroxyapatite
• direct electrochemistry
• biosensor

A novel biosensor based on Fe-doped hydroxyapatite (Fe-HA) nanoparticles and tyrosinase has been developed for the detection of L-tyrosine. Nanostructured Fe-HA was synthesized by a simple microwave irradiation method, and its phase formation, morphology and magnetic property were examined by powder X-ray diffraction (XRD), transmission electron microscopy (TEM) and vibrating sample magnetometer (VSM). Electrochemical performance of the nano Fe-HA/tyrosinase modified glassy carbon electrode (GCE) for detection of L-tyrosine was investigated by cyclic voltammetry (CV) and amperometric methods. The fabricated biosensor exhibited a linear response to L-tyrosine over a wide concentration range of 1.0 x 10(-7) to 1.0 x 10(-5) M with a detection limit of 245 nM at pH 7.0. In addition, the fabricated sensor showed an excellent selectivity, good reproducibility, long-term stability and anti-interference towards the determination of L-tyrosine.

• 出版日期2014-2-1