alpha(2)-Adrenoceptor agonist dexmedetomidine protects septic acute kidney injury through increasing BMP-7 and inhibiting HDAC2 and HDAC5

作者：Hsing Chung Hsi; Lin Chiou Feng; So Edmund; Sun Ding Ping; Chen Tai Chi; Li Chien Feng; Yeh Ching Hua^*

来源：American Journal of Physiology - Renal Fluid and Electrolyte Physiology, 2012, 303(10): F1443-F1453.

DOI：10.1152/ajprenal.00143.2012

• dexmedetomidine
• sepsis
• acute kidney injury
• bone morphogenetic protein-7
• histone deacetylase 2
• histone deacetylase 5

Hsing CH, Lin CF, So E, Sun DP, Chen TC, Li CF, Yeh CH. alpha(2)-Adrenoceptor agonist dexmedetomidine protects septic acute kidney injury through increasing BMP-7 and inhibiting HDAC2 and HDAC5. Am J Physiol Renal Physiol 303: F1443-F1453, 2012. First published August 29, 2012; doi:10.1152/ajprenal.00143.2012.-Bone morphogenetic protein (BMP)-7 protects sepsis-induced acute kidney injury (AKI). Dexmedetomidine (DEX), an alpha(2)-adrenoceptor (alpha(2)-AR) agonist, has anti-inflammatory effects. We investigated the protective effects of DEX on sepsis-induced AKI and the expression of BMP-7 and histone deacetylases (HDACs). In vitro, the effects of DEX or trichostatin A (TSA, an HDAC inhibitor) on TNF-alpha, monocyte chemotactic protein (MCP-1), BMP-7, and HDAC mRNA expression in LPS-stimulated rat renal tubular epithelial NRK52E cells, was determined using real-time PCR. In vivo, mice were intraperitoneally injected with DEX (25 mu g/kg) or saline immediately and 12 h after cecal ligation and puncture (CLP) surgery. Twenty-four hours after CLP, we examined kidney injury and renal TNF-alpha, MCP-1, BMP-7, and HDAC expression. Survival was monitored for 120 h. LPS increased HDAC2, HDAC5, TNF-alpha, and MCP-1 expression, but decreased BMP-7 expression in NRK52E cells. DEX treatment decreased the HDAC2, HDAC5, TNF-alpha, and MCP-1 expression, but increased BMP-7 and acetyl histone H3 expression, whose effects were blocked by yohimbine, an alpha(2)-AR antagonist. With DEX treatment, the LPS-induced TNF-alpha expression and cell death were attenuated in scRNAi-NRK52E but not BMP-7 RNAi-NRK52E cells. In CLP mice, DEX treatment increased survival and attenuated AKI. The expression of HDAC2, HDAC5, TNF-alpha, and MCP-1 mRNA in the kidneys of CLP mice was increased, but BMP-7 was decreased. However, DEX treatment reduced those changes. DEX reduces sepsis-induced AKI by decreasing TNF-alpha and MCP-1 and increasing BMP-7, which is associated with decreasing HDAC2 and HDAC5, as well as increasing acetyl histone H3.

• 出版日期2012-11
• 单位河北医科大学