Use of Lactobacillus rossiae DC05 for bioconversion of the major ginsenosides Rb1 and Re into the pharmacologically active ginsenosides C-K and Rg2

作者：Huq Md Amdadul; Kim Yeon Ju; Min Jin Woo; Bae Kwi Sik; Yang Deok Chun^*

来源：Food Science and Biotechnology, 2014, 23(5): 1561-1567.

DOI：10.1007/s10068-014-0212-3

• major ginsenosides
• bioconversion
• Lactobacillus rossiae

Rb1 and Re are the major ginsenosides in protopanaxadiol and protopanaxatriol with contents of 38.89 and 13.34%, respectively. beta-Glucosidase-producing food grade Lactobacillus rossiae DC05 was isolated from kimchi using esculin-MRS agar and an enzyme of L. rossiae DC05 was used for bioconversion of the major ginsenosides Rb1 and Re. Strain DC05 showed strong activity in converting ginsenosides Rb1 and Re into the minor ginsenosides compound-K and Rg2, respectively. Within 4 days, 100% of ginsenoside Rb1 was decomposed and converted into C-K, while 85% of Re was decomposed and converted into Rg2 after 6 days of incubation. The biosynthesis rate of ginsenoside C-K was 72.88%, and the biosynthesis rate of Rg2 was 53.94%. Strain DC05 hydrolyzed ginsenosides Rb1 and Re along the pathway Rb1 -%26gt; Rd -%26gt; F2 -%26gt; CK and the pathway Re -%26gt; Rg2, respectively. The optimum temperature and pH of the enzyme were 30A degrees C and 7.0, respectively.

• 出版日期2014-10