Acaricidal activity of petroleum ether extract of neem (Azadirachta indica) oil and its four fractions separated by column chromatography against Sarcoptes scabiei var. cuniculi larvae in vitro

作者：Deng, Yunxia; Shi, Dongxia; Yin, Zhongqiong^*; Guo, Jianhong; Jia, Renyong; Xu, Jiao; Song, Xu; Lv, Cheng; Fan, Qiaojia; Liang, Xiaoxia; Shi, Fei; Ye, Gang; Zhang, Wei

来源：Experimental Parasitology, 2012, 130(4): 475-477.

DOI：10.1016/j.exppara.2012.02.007

• Sarcoptes scabiei
• Acaricidal activity
• Neem oil
• In vitro

The petroleum ether extract of neem oil and its four fractions separated by column chromatography was diluted at different concentrations with liquid paraffin. The acaricidal bioassay was conducted using a dipping method. The results indicated that the median lethal concentration (LC50) of the petroleum ether extract (at the concentration of 500.0 ml/l) was 70.9 ml/l, 24 h after treatment. At concentrations of 500.0, 250.0, 125.0, 62.5 and 31.2 ml/l, the median lethal times (LT50) of the petroleum ether extract were 8.7, 8.8, 10.8, 11.5 and 13.1 h, respectively. Thin-layer chromatography (TLC) showed that the petroleum ether extract of neem oil separated into four fractions (F1-F4). Acaricidal activity of 68.3% and 100.0% in the F2 and F4 was confirmed. These results suggest that petroleum ether extracts of neem oil and its four fractions possess useful acaricidal activity in vitro.

• 出版日期2012-4
• 单位中国农业科学院兰州兽医研究所; 四川农业大学