Gas Chromatography-Mass Spectrometric Analysis of Nematicidal Essential Oil of Valeriana amurensis P Smirn ex Kom (Valerianaceae) Roots and its Activity against Heterodera avenae

作者：Li, Yun Chao; Ji, Hua; Li, Hong Tao^*

来源：Tropical Journal of Pharmaceutical Research, 2015, 14(9): 1673-1678.

DOI：10.4314/tjpr.v14i9.18

• Valeriana amurensis
• Heterodera avenae
• Nematicidal activity
• Isovaleric acid
• 3-Methylvaleric acid
• Essential oil
• Cereal cyst nematodes

Purpose: To investigate the chemical composition and nematicidal activity of the essential oil of Valeriana amurensis roots against cereal cyst nematodes (Heterodera avenae). @@@ Methods: The essential oil of V. amurensis roots was obtained by hydrodistillation and analyzed by gas chromatography (GC) and gas chromaotography-mass spectrometry (GC-MS). The nematicidal activity of the essential oil and its major constituents was determined against second stage juveniles of H. avenae. @@@ Results: A total of 33 components of the essential oil were identified. The major constituents were bornyl acetate (12.5 %), patchoulol (11.6 %), caryophyllene (8.2 %), 3-methylvaleric acid (7.3 %) and isovaleric acid (6.5 %). The essential oil exhibited nematicidal activity against H. avenae with a medium lethal concentration (LC50) value of 311.6 mu g/mL. The major constituents, isovaleric acid and 3-methylvaleric acid, exhibited nematicidal activity against H. avenae with LC50 of 218.2 and 683.8 mu g/mL, respectively. @@@ Conclusion: The study indicates that the essential oil of V. amurensis roots and its two major constituents, isovaleric acid and 3-methylvaleric acid, have a potential to be developed to natural nematicides for the control of cereal cyst nematodes.

• 出版日期2015-9
• 单位河北省农林科学院