科研之友
微信
新浪微博
Facebook
分享链接
摘要
A reliable chromatographic method for the determination of soy isoflavones (genistein, daidzein and glycitein) using a coulometric detection has been developed and applied to analyse plasma of postmenopausal women The chromatographic separation was performed on a C18 reversed phase column with a mobile phase composed of acetonitrile-phosphate buffer mixture. Coulometric detection was carried out at +0.500 V A careful and rapid solid phase extraction procedure on hydrophilic/lipophilic cartridges was chosen for plasma sample purification with and without hydrolysis obtaining good extraction yield values for all the analytes (>90 0%). The enzymatic hydrolysis step was necessary for the determination of the total amount of soy isoflavones The limit of quantitation was 05 ng mL(-1) for genistein and 025 ng mL(-1) for daidzein and glycitein. The method was found to be precise and accurate Thus, the proposed method is su
- 出版日期2010-11-2
