Antigen presentation by small intestinal epithelial cells uniquely enhances IFN-gamma secretion from CD4(+) intestinal intraepithelial lymphocytes

作者：Hatano Ryo; Yamada Kiyoshi; Iwamoto Taku; Maeda Nana; Emoto Tetsuro; Shimizu Makoto; Totsuka Mamoru^*

来源：Biochemical and Biophysical Research Communications, 2013, 435(4): 592-596.

DOI：10.1016/j.bbrc.2013.05.024

• Small intestinal epithelial cells
• CD4(+) intestinal intraepithelial lymphocytes
• Interferon-gamma
• Major histocompatibility complex class II molecule
• Antigen specific activation

Small intestinal epithelial cells (sIECs) express major histocompatibility complex class II molecules even in a normal condition, and are known to function as antigen presenting cells (APCs) at least in vitro. These findings raised the possibility that sIECs play an important role in inducing immune responses against luminal antigens, especially those of intestinal intraepithelial lymphocytes (IELs) and lamina propria lymphocytes (LPLs). We herein showed that antigenic stimulation with sIECs induced markedly greater secretion of interferon-gamma (IFN-gamma) by CD4(+) IELs, but not interleukin (IL)-4, IL-10 and IL-17 although the proliferative response was prominently lower than that with T cell-depleted splenic APCs. In contrast, no enhanced IFN-gamma secretion by CD4(+) LPLs and primed splenic CD4(+) T cells was observed when stimulated with sIECs. Taken together, these results suggest that sIECs uniquely activate CD4(+) IELs and induce remarkable IFN-gamma secretion upon antigenic stimulation in vivo.

• 出版日期2013-6-14