A novel role for cardiac ankyrin repeat protein Ankrd1/CARP as a co-activator of the p53 tumor suppressor protein

作者：Kojic Snezana^*; Nestorovic Aleksandra; Rakicevic Ljiljana; Belgrano Anna; Stankovic Marija; Divac Aleksandra; Faulkner Georgine

来源：Archives of Biochemistry and Biophysics, 2010, 502(1): 60-67.

DOI：10.1016/j.abb.2010.06.029

• Ankrd1/CARP
• p53
• Transcription factor
• Skeletal muscle
• Myogenesis

The muscle ankyrin repeat protein (MARP) family member Ankrd1/CARP is a part of the titin-mechanosensory signaling complex in the sarcomere and in response to stretch it translocates to the nucleus where it participates in the regulation of cardiac genes as a transcriptional co-repressor. Several studies have focused on its structural role in muscle, but its regulatory role is still poorly understood To gain more insight into the regulatory function of Ankrd1/CARP we searched for transcription factors that could interact and modulate its activity. Using protein array methodology we identified the tumor suppressor protein p53 as an Ankrd1/CARP interacting partner and confirmed their interaction both in vivo and in vitro We demonstrate a novel role for Ankrd1/CARP as a transcriptional co-activator, moderately up regulating p53 activity Furthermore, we show that p53 operates as an upstream effector of Ankrd1/CARP, by up regulating the proximal ANKRD1 promoter Our findings suggest that, besides acting as a transcriptional co-repressor, Ankrd1/CARP could have a stimulatory effect on gene expression in cultured ske

• 出版日期2010-10-1