N-hydroxysuccinimidyl p-methoxybenzoate as suitable derivative reagent for isotopic dilution assay of biogenic amines in food

作者：Mazzotti Fabio^*; Di Donna Leonardo; Napoli Anna; Aiello Donatella; Siciliano Carlo; Athanassopoulos Constantinos M; Sindona Giovanni

来源：Journal of Mass Spectrometry, 2014, 49(9): 802-810.

DOI：10.1002/jms.3417

• biogenic amines
• N-hydroxysuccinimidyl ester
• LC-MS
• MS-MRM
• MALDI
• stable isotope-coded derivatization (ICD)

We present a simple methodology for the simultaneous identification and determination of biogenic amines in food matrices, based on the use of a stable isotope-coded derivatization and liquid chromatography tandem mass spectrometry. The tagging reagent is N-hydroxysuccinimidyl ester of d(0)/d(4)-4-methoxybenzoic acid (d(0)/d(4)-4-MBA-OSu) which mainly functionalizes primary amines. The identification and structural characterization of tagged biogenic amines were exploited by matrix-assisted laser desorption/ionization-mass spectrometry (MS) and MS/MS. Multiple-reaction monitoring has been applied in the assay of biogenic amines in different foodstuffs, providing a method whose reliability is confirmed by the values of accuracy (12%) and by the calculated analytical parameters.

• 出版日期2014-9