Application of quantitative H-1-NMR method to determination of gentiopicroside in Gentianae radix and Gentianae scabrae radix

作者：Tanaka Rie; Hasebe Yuko; Nagatsu Akito^*

来源：Journal of Natural Medicines, 2014, 68(3): 630-635.

DOI：10.1007/s11418-014-0833-0

• Gentiopicroside
• Quantitative H-1-NMR
• Gentianae radix
• Gentianae scabrae radix
• Reagent purity

A quantitative H-1-NMR method (qHNMR) was used to measure gentiopicroside content in Gentianae radix and Gentianae scabrae radix. Gentiopicroside is a major component of Gentianae radix and Gentianae scabrae radix. The purity of gentiopicroside was calculated from the ratio of the intensity of the H-3 signal at delta 7.44 ppm or the H-8 signal at delta 5.78 ppm in methanol-d (4) of gentiopicroside to that of a hexamethyldisilane (HMD) signal at 0 ppm. The concentration of HMD was corrected with SI traceability by using potassium hydrogen phthalate of certified reference material (CRM) grade. As a result, the gentiopicroside content in two lots of Gentianae radix as determined by qHNMR was found to be 1.76 and 2.17 %, respectively. The gentiopicroside content in two lots of Gentianae scabrae radix was 2.73 and 3.99 %, respectively. We demonstrated that this method is useful for the quantitative analysis of crude drugs.

• 出版日期2014-7