Analysis of Expressed Sequence Tags from the Wood-Decaying Fungus Fomitopsis palustris and Identification of Potential Genes Involved in the Decay Process

作者：Karim Nurul; Shibuya Hajime; Kikuchi Taisei^*

来源：Journal of Microbiology and Biotechnology, 2011, 21(4): 347-358.

DOI：10.4014/jmb.1010.10048

• ESTs
• Fomitopsis palustris
• brown-rot fungus
• GH family 45 endoglucanase
• Gene Ontology

Fomitopsis palustris, a brown-rot basidiomycete, causes the most destructive type of decay in wooden structures. In spite of its great economic importance, very little information is available at the molecular level regarding its complex decay process. To address this, we generated over 3,000 expressed sequence tags (ESTs) from a cDNA library constructed from F. palustris. Clustering of 3,095 high-quality ESTs resulted in a set of 1,403 putative unigenes comprising 485 contigs and 918 singlets. Homology searches based on BlastX analysis revealed that 78% of the E palustris unigenes had a significant match to proteins. deposited in the nonredundant databases. A subset of E palustris unigenes showed similarity to the carbohydrate-active enzymes (CAZymes), including a range of glycosyl hydrolase (CH) family proteins. Some of these CAZyme-encoded genes were previously undescribed for F. palustris but predicted to have potential roles in biodegradation of wood. Among them, we identified and characterized a gene (FpCe145A) encoding the CH family 45 endoglucanase. Moreover, we also provided functional classification of 473 (34%) of E palustris unigenes using the Gene Ontology hierarchy. The annotated EST data sets and related analysis may be useful in providing an initial insight into the genetic background of E palustris.

• 出版日期2011-4