A three-stage culture process for improved exopolysaccharide production by Tremella fuciformis

作者：Zhu Hu^*; Tian Baozhen; Liu Wei; Zhang Shuaishuai; Cao Chunxu; Zhang Yan; Zou Weisheng

来源：Bioresource Technology, 2012, 116: 526-528.

DOI：10.1016/j.biortech.2012.03.117

• Three-stage culture
• Exopolysaccharide
• Orbital and reciprocating shakers
• Tremella fuciformis spore

Tremella fuciformis produces several bioactive secondary metabolites including exopolysaccharides. Cultivation of the fungus was carried out in a three-stage process consisting of a 1.5-day cultivation with orbital shaking at 200 rpm, a 1.5-day cultivation with reciprocal shaking at 200 strokes, and a 1.5-day cultivation with orbital shaking at 200 rpm. Exopolysaccharide production and specific production rate reached 5.80 g L-1 and 0.15 d(-1), respectively, which is an increase of 260% and 200% compared with the corresponding values for fermentations with orbital shaking only, and of 243% and 150% compared with the corresponding values for fermentations with reciprocal shaking only. The three-stage culture method is time-saving and easy to operate.

• 出版日期2012-7
• 单位中国石油大学（华东）