Phenotypic and molecular characterization of Arcanobacterium haemolyticum isolated from clinical samples

作者：Garcia de la Fuente Celia^*; Ruiz de Alegria Carlos; Eliecer Cano Maria; Belen Campo Ana; Unda Fabian; Agueero Jesus; Martinez Martinez Luis

来源：Diagnostic Microbiology and Infectious Disease, 2012, 72(1): 1-7.

DOI：10.1016/j.diagmicrobio.2011.09.005

• Arcanobacterium haemolyticum
• Pharyngitis
• Rash

The aim of this study was to assess the phenotypic and genotypic diversity of 56 Arcanobacterium haemolyticum isolates isolated from 51 patients attending primary health care centres and emergency units in the health area of Santander (Cantabria, northern Spain). Phenotypic characterization was based on morphological, biochemical, and antigenic tests. Species identification was confirmed by amplification and sequencing of the 16S rDNA gene. Antimicrobial susceptibility testing was determined by microdilution following the Clinical and Laboratory Standards Institute recommendations for coryneform bacteria. Genetic diversity was evaluated using BOX-PCR and pulsed-field gel elcctrophoresis. Eighty percent of the isolates had an identical BOX-PCR pattern, suggesting the spread of a single clone. The present report provides extensive information on the phenotypic and genotypic characterization of A. haetnolyticum.

• 出版日期2012-1