Developmental Cycle and Genome Analysis of Protochlamydia massiliensis sp nov a New Species in the Parachlamydiacae Family

作者：Benamar Samia; Khalil Jacques Y Bou; Blanc Tailleur Caroline; Bilen Melhem; Barrassi Lina; La Scola Bernard^*

来源：Frontiers in Cellular and Infection Microbiology, 2017, 7: 385.

DOI：10.3389/fcimb.2017.00385

• Chlamydiae
• Vermamoeba vermiformis
• co-culture
• CRISPR
• Protochlamydia massiliensis

Amoeba-associated microorganisms (AAMs) are frequently isolated from water networks. In this paper, we report the isolation and characterization of Protochlamydia massiliensis, an obligate intracellular Gram-negative bacterium belonging to the Parachlamydiaceae family in the Chlamydiales order, from a cooling water tower. This bacterium was isolated on Vermamoeba vermiformis. It has a multiple range of hosts among amoeba and is characterized by a typical replication cycle of Chlamydiae with a particularity, recently shown in some chlamydia, which is the absence of inclusion vacuoles in the V. vermiformis host, adding by this a new member of Chlamydiae undergoing developmental cycle changes in the newly adapted host V. vermiformis. Draft genome sequencing revealed a chromosome of 2.86 Mb consisting of four contigs and a plasmid of 92 Kb.

• 出版日期2017-8-31