A Novel In Vitro Assay to Assess Phosphorylation of 3 '-[F-18]fluoro-3 '-Deoxythymidine

作者：Guo Ning^*; Xie Jingping; Manning H Charles; Deane Natasha G; Ansari M Sib; Coffey Robert J; Gore John; Price Ronald R; Baldwin Ronald M; McIntyre J Oliver

来源：Molecular Imaging and Biology, 2011, 13(2): 257-264.

DOI：10.1007/s11307-010-0351-8

• 3 '-[F-18]fluoro-3 '-deoxythymidine ([F-18]FLT)
• Thymidine kinase 1
• Positron emission tomography (PET)
• PET tracers
• Molecular imaging

The authors discuss one type of general forward-backward stochastic differential equations (FBSDEs) with It's stochastic delayed equations as the forward equations and anticipated backward stochastic differential equations as the backward equations. The existence and uniqueness results of the general FBSDEs are obtained. In the framework of the general FBSDEs in this paper, the explicit form of the optimal control for linearquadratic stochastic optimal control problem with delay and the Nash equilibrium point for nonzero sum differential games problem with delay are obtained.
The intrinsic F-18 radioactivity was used to quantify both substrate and phosphorylated products using a rapid thin layer chromatography method. Phosphorylation kinetics of [F-18]FLT in SW480 and DiFi tumor cell lysates and cellular uptake were measured.
The apparent Michaelis-Menten kinetic parameters for [F-18]FLT are and V (max) = 7.4 pmol min(-1) per 1 x 10(6) cells with similar to 2-fold higher TK-1 activity in DiFi versus SW480 lysates.
The apparent K (m) of [F-18]FLT was comparable to the value reported with purified recombinant TK-1. The uptake of [F-18]FLT by SW480 cells is inhibited by nitrobenzylthioinosine or dipyridamole indicating that uptake is mediated predominantly by the equilibrative nucleoside transporters in these tumor cells.

• 出版日期2011-4