Aeromonas hydrophila strains as biocatalysts for transglycosylation

作者：Nobile Matias; Terreni Marco; Lewkowicz Elizabeth^*; Iribarren Adolfo M

来源：Biocatalysis and Biotransformation, 2010, 28(5-6): 395-402.

DOI：10.3109/10242422.2010.538949

• Aeromonas hydrophila
• microbial transglycosylation
• whole cell biocatalysts
• ribavirin
• didanosine

Microbial transglycosylation is useful as a green alternative in the preparation of purine nucleosides and analogues, especially for those that display pharmacological activities. In a search for new transglycosylation biocatalysts, two Aeromonas hydrophila strains were selected. The substrate specificity of both micro-organisms was studied and, as a result, several nucleoside analogues have been prepared. Among them, ribavirin, a broad spectrum antiviral, and the well-known anti HIV didanosine, were prepared, in 77 and 62% yield using A. hydrophila CECT 4226 and A. hydrophila CECT 4221, respectively. In order to scale-up the processes, the reaction conditions, product purification and biocatalyst preparation were analyzed and optimized.

• 出版日期2010-12