Nanozeolite-assembled interface towards sensitive biosensing

作者：Zhou Xiaoqin; Yu Tao; Zhang Yahong^*; Kong Jilie; Tang Yi; Marty Jean Louis; Liu Baohong

来源：Electrochemistry Communications, 2007, 9(7): 1525-1529.

DOI：10.1016/j.elecom.2007.02.018

• nanozeolite
• layer-by-layer
• biointerface
• electrochemistry
• sensor

A biocompatible surface has been constructed on the electrode surface via layer-by-layer assembly of beta-nanozeolites and polydiallyldimethylammonium (PDDA) for the adsorption of enzymes towards sensitive biosensing. The film assembly process and enzyme adsorption were monitored by Quartz Crystal Microbalance measurements. The nanozeolite film exhibited an amazing adsorption capacity (about 350 mg g(-1)) for tyrosinase as a model enzyme. The tyrosinase biosensor showed a high sensitivity (400 mu A mM(-1)), short response time (reaching 95% within 5 s), broad linear response range from 10 nM to 18 mu M, very low detection limit (0.5 nM) and high operational and storage stability (more than 2 months). The apparent Michaelis-Menten constant K-M(app) was calculated to be 24 mu M using phenol as the substrate. The assembly-controlled nanozeolite film could provide a biocompatible surface for the interaction study between enzymes and target molecules.

• 出版日期2007-7
• 单位复旦大学