Fast analysis of caffeine in beverages and drugs by paper spray tandem mass spectrometry

作者：Taverna Domenico; Di Donna Leonardo; Bartella Lucia; Napoli Anna; Sindona Giovanni; Mazzotti Fabio^*

来源：Analytical and Bioanalytical Chemistry, 2016, 408(14): 3783-3787.

DOI：10.1007/s00216-016-9468-1

• Caffeine
• Paper spray
• Mass spectrometry
• Multiple reaction monitoring
• Quantitative assay

A simple and fast method based on paper spray mass spectrometry for the determination of caffeine in commercial beverages and drugs has been developed; the analyses were carried out in MRM mode, monitoring the transitions m/z 195 -> m/z 138 for caffeine and m/z 198 -> m/z 140 for the labeled internal standard. To verify the reliability of the proposed approach, a spiked sample (soda drink and paracetamol tablet) with a known amount of caffeine has been prepared and analyzed by PS-MS, providing accuracy values about 100 %; the LOQ and LOD values were calculated at 1.2 and 1.6 mu g/mL, respectively. Both beverages and drugs were also analyzed with the classic analytical method based on LC-UV measurements, showing consistent results between the two approaches, thus confirming the reliability of the developed ambient MS determination.

• 出版日期2016-5