科研之友
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摘要
Ca2 is a highly versatile second messenger that plays a key role in the regulation of numerous cell processes. One-way cells ensure the specificity and reliability of Ca2 signals is by organizing them spatially in the form of waves that propagate throughout the cell or within a specific subcellular region. In non-excitable cells, the inositol 1,4,5-trisphosphate receptor (IP3R) is responsible for the release of Ca2 from the endoplasmic reticulum. The spatial aspect of the Ca2 signal depends on the organization of various elements of the Ca2 signaling toolkit and varies from tissue to tissue. Ca2 is implicated in many of endothelium functions that thus depend on the versatility of Ca2 signaling. In the present study, we showed that the disruption of caveolae microdomains in bovine aortic endothelial cells (BAEC) with methyl-3-cyclodextrin was not sufficient to disorganize the propagation of Ca2 waves when the cells were stimulated with ATP or bradykinin. However, disorganizing microfilaments with latrunculin B and microtubules with colchicine both prevented the formation of Ca2 waves. These results suggest that the organization of the Ca2 waves mediated by IP3R channels does not depend on the integrity of caveolae in BAEC, but that microtubule and microfilament cytoskeleton assembly is crucial. J. Cell. Biochem. 106: 344-352, 2009.
- 出版日期2009-2-1
