Deleted in Esophageal Cancer 1 (DEC1) Is Down-Regulated and Contributes to Migration in Head and Neck Squamous Cell Carcinoma Cell Lines

作者：Kunimoto Yasuomi^*; Nakano Seiji; Kataoka Hideyuki; Shimada Yutaka; Oshimura Mitsuo; Kitano Hiroya

来源：ORL: Journal for Oto-Rhino-Laryngology and Its Related Specialties , 2011, 73(1): 17-23.

DOI：10.1159/000320997

• Head and neck squamous cell carcinoma
• DEC1
• Tumor suppressor gene
• Histone H3 K27 trimethylation
• Migration
• Invasion

Background: Previous studies have shown that the expression of Deleted in Esophageal Cancer 7 (DEC1) is significantly reduced in esophageal squamous cell carcinoma. Patients with head and neck squamous cell carcinoma (HNSCC) often develop esophageal carcinomas. Materials and Methods: We analyzed the expression of DEC1 and histone modifications in HNSCC cell lines. The motility and invasive ability of the HNSCC cell lines were also studied. Results: Of 18 cell lines, 12(66.7%) showed down-regulation of DEC1. Chromatin immunoprecipitation assays indicated that H3 K27 trimethylation levels in the DEC1-down-regulated cell lines were greater than that in the DEC1-expressed cell lines. Migration assays showed that the DEC1-down-regulated cell lines tended to be more motile than the DEC1-expressed cell lines. Conclusion: DEC1 is down-regulated and tends to contribute to the migration ability of HNSCC cell lines. In addition, H3 K27 trimethylation potentially plays an important role in the regulation of DEC1 expression.

• 出版日期2011