Analysis of the promoter region of the gene LIP1 encoding triglyceride lipase from Fusarium graminearum

作者：Feng Jie; Bhadauria Vijai; Liu Guosheng; Selvaraj Gopalan; Hughes Geoffrey R; Wei Yangdou^*

来源：Microbiological Research, 2011, 166(8): 618-628.

DOI：10.1016/j.micres.2010.12.002

• Gene regulation
• Green fluorescent protein
• Fatty acid responsive element
• Fusarium head blight

Triglyceride lipases catalyze the reversible degradation of glycerol esters with long-chain fatty acids into fatty acids and glycerol. In silico analysis of 5'-end flanking sequence of the gene LIP1 encoding a triglyceride lipase from the wheat head blight pathogen Fusarium graminearum revealed the presence of several cis-regulatory elements. To delineate the function of these regulatory elements, we constructed a series of deletion mutants in the LIP1 promoter region fused to the open reading frame of a green fluorescent protein (GFP) and assayed the promoter activity. Analysis of GFP expression levels in mutants indicated that a 563-bp promoter sequence was sufficient to drive the expression of LIP1 and regulatory elements responsible for the gene induction were located within the 563-372 bp region. To further investigate the regulatory elements, putative cis-acting elements spanned within the 563-372 bp region were mutated using a targeted mutagenesis approach. A CCAAT box, a CreA binding site, and a fatty acid responsive element (FARE) were identified and confirmed to be required for the basal expression of LIP1, glucose suppression and fatty acid induction, respectively.

• 出版日期2011
• 单位Saskatchewan; Saskatoon