科研之友
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摘要
Objective To establish a simple, effective and stable rabbit brain recovery method on electrically induced fibrillation cardiac arrest (CA).Methods Twenty New Zealand male rabbits were used to set up a rabbit ventricular fibrillation induced CA model by introducing alternating current on the surface under the chest wall and endocardium and then start cardiopulmonary resuscitation (CPR) 4 minutes after CA .The operation time before CPR , restoration of spontaneous circulation ( ROSC ) time, spontaneous circulation response rates , 24 h, 72 h survival rate of the 20 rabbits, the defibrillation frequency , the administration frequency , TOP and TROSC and the apoptotic cells of cerebral cortex on ROSC 72 h have been recorded .For comparison, 10 other rabbits were taken for the surgical operation without fibrillation or CPR .Results ①Cardiac arrest was successfully induced in all 20 rabbits. Incidence of ventricular fibrillation was 100% after electrical stimulation .ROSC rate was 80% and 24 h, 72 h survival rate was 50% (10/20) and 30% (6/20), respectively.The defibrillation frequency was 1.20 ±0.89.The frequency of intravenous injection of adrenaline was 1.60 ±0.75.TOP and TROSC were ( 79 ±25 ) minutes and ( 268 ±33 ) seconds , respectively .②The plasma enzyme specificity enolization of neurons ( NSE) levels increased significantly after building the model .It fell back slightly within 72 hours, but still higher than the level before the model was built (P <0.05).③Apoptosis of nerve cells appeared more in brain cortex after ROSC 72 h than that in the control group (P <0.05).④The lowest NDS was at 3 hours after ROSC , while the NDS increased gradually .The NDS of ROSC 72 h was 72.67 ±9.11 (n=6).Conclusion The rabbit by chest and endocardium induced fibrillation CA model was easy to operate , had stable results and a high recovery rate .It is a relatively ideal cardiopulmonary cerebral resuscitation research model .
- 出版日期2014
- 单位中国人民解放军第二军医大学
