Microscopic Imaging of Glass Surfaces under the Effects of Desorption Electrospray Ionization

作者：Wood Michael C; Bu**y Devin K; Farnsworth Paul B^*

来源：Analytical Chemistry, 2009, 81(15): 6407-6415.

DOI：10.1021/ac9008868

Microscopic imaging techniques were used to study sample removal from a glass surface by desorption electrospray ionization (DESI). Changes in fluorescence or absorption images of organic dyes were used to track the impact of the DESI spray on a smooth surface. Time-resolved images revealed rapid removal of the dye from an elliptical region impacted directly by the spray, followed by slower redistribution of the sample across the surface by flowing solvent and/or spray gases. The persistence of a mass spectrometric signal long after the dye was removed from the central impact region indicated that ions were being produced using processes other than impact of the spray on the surface. Placement of a barrier between the DESI spray tip and the mass spectrometer inlet provided additional evidence that ions were being produced at the ends of rivulets streaming away from the central impact region.

• 出版日期2009-8-1