Molecular cloning and characterization of a new peroxidase gene (OvRCI) from Orychophragmus violaceus

作者：Dai Qilin; Wang Jin; Feng Bin; Liu Tingting; Chen Chen; Lin Honghui^*; Du Shizhang

来源：African Journal of Biotechnology, 2009, 8(23): 6511-6517.

• Orychophragmus violaceus
• OvRCI
• quantitative real-time PCR

A new peroxidase gene from Orychophragmus violaceus was cloned. The full-length cDNA of O. violaceus peroxidase gene (OvRCI, GenBank. Acc. No. AY428037) was 1220 bp and contained an 1128 bp open reading frame encoding a protein of 375 amino acids. Homology analysis and molecular modeling revealed that OvRCI strongly resembled other peroxidase genes. Quantitative real-time PCR analysis revealed that it was a constitutively salt-inducible gene and its transcript level was most abundant after 24 h treatment with 200 mmol.L(-1) sodium chloride. Our studies suggested that OvRCI was a new member of the family of recently cloned peroxidase genes.

• 出版日期2009-12-1
• 单位四川大学; 西南科技大学; 绵阳师范学院; 复旦大学