A two-photon off-on fluorescence probe for imaging thiols in live cells and tissues

作者：Zhu, Xinyue; Li, Yan; Zan, Wenyan; Zhang, Jianjian; Chen, Zhenjie; Liu, Xiaoyan; Qi, Fengchao; Yao, Xiaojun; Zhang, Xiaoyu; Zhang, Haixia^*

来源：Photochemical and Photobiological Sciences, 2016, 15(3): 412-419.

DOI：10.1039/c5pp00468c

• ABSORPTION CROSS-SECTIONS
• LIVING CELLS
• SELECTIVE DETECTION
• HYDROGEN-SULFIDE
• HOMOCYSTEINE
• CYSTEINE
• GLUTATHIONE
• PHTHALOCYANINE
• CHROMOPHORES
• BIOTHIOLS

A two-photon (TP) fluorescence imaging probe (Z1) was designed to detect biothiols through a photo-induced electron transfer pathway utilizing N-butyl-naphthalimide as the fluorophore and 2,4-dinitrobenzene-sulfonyl as the responsive group, which were linked together by piperazine. The synthesized Z1 displayed high selectivity to biothiols, significant fluorescence off-on properties, and a marked two-photon absorption cross section (delta = 110 GM). Moreover, Z1 showed good biocompatibility and insensitivity toward changes in the biologically relevant pH range (7.2-8.4), which enabled the utilization of Z1 to monitor biothiol levels not only in live cells but also in tissues at depths of 50-250 mu m.

• 出版日期2016
• 单位兰州大学