Application of mitochondrial DNA polymorphism to Meloidogyne molecular population biology

作者：Hyman BC; Whipple LE

来源：Journal of Nematology, 1996, 28(3): 268-276.

• AFLP
• genetics
• mitochondrial DNA
• molecular biology
• molecular markers
• nuclear DNA
• PCR
• RAP
• RFLP
• root-knot nematode
• VNTR

Recent advances in molecular biology have enabled the genotyping of individual nematodes, facilitating the analysis of genetic variability within and among plant-pathogenic nematode isolates. This review first describes representative examples of how RFLP, RAPD, AFLP, and DNA sequence analysis have been employed to describe populations of several phytonematodes, including the pinewood, burrowing, root-knot, and cyst nematodes. The second portion of this paper evaluates the utility of a size-variable mitochondrial DNA locus to examine the generic structure of Meloidogyne isolates using two alternate methodologies, variable number tandem repeat (VNTR) and repeat associated polymorphism (RAP) analysis. VNTR analysis has revealed genetic variation among individual nematodes, whereas RAP may provide useful markers for species and population differentiation.

• 出版日期1996-9