Application of tissue microarray: Evaluation of the expression of S-100-positive dendritic cells, tumor suppressor gene p63 and tissue inhibitor of metalloproteinase-1 in laryngeal carcinoma

作者：Dong, P^*; Li, XY; Zhu, ZH; Yu, ZW; Lu, GZ; Sun, ZF; Wang, S

来源：Acta Oto-Laryngologica, 2004, 124(10): 1204-1207.

DOI：10.1080/00016480410017161

• dendritic cell
• immunohistochemistry
• laryngeal carcinoma
• p63 gene
• prognosis
• tissue inhibitor of metalloproteinase-1
• tissue chip technology
• tissue microarray

Objective - To evaluate the predictive role of S100-positive dendritic cells, tissue inhibitor of metalloproteinase-1 (TIMP1) and p63 gene in laryngeal carcinoma using a tissue chip.
Material and Methods - The expression of dendritic cells, TIMP1 and p63 gene in a series of 85 primary laryngeal carcinoma patients who had undergone operations in our hospital between 1992 and 2000 was studied immunohistochemically using the streptavidin - biotin peroxidase-conjugated method.
Results - Some cases were lost or showed no tumor tissue in the tissue chip. The rate of expression of dendritic cells was 59.5% and there were significant differences in expression between the differential degrees of laryngeal squamous carcinoma and the different clinical stages (p< 0.05). The basal membrane and extracellular matrix of the specimens were strongly stained by TIMP1 in 55.7% of cases. There were statistically significant correlations between TIMP1 protein expression and early- and advanced-stage tumors, lymph node metastasis and 3-year survival rate (p< 0.05). No statistically significant correlations were found between p63 protein expression and any of the clinical parameters.
Conclusions - The tissue microarray technique used in this study is quick, inexpensive and very consistent. It is suggested that TIMP1 and S100 should be used as clinical discriminators of laryngeal carcinoma.

• 出版日期2004-12
• 单位上海交通大学