Modifications of Commercial Toxigenic Clostridium difficile PCR Resulting in Improved Economy and Workflow Efficiency

作者:Munson Erik*; Bilbo Dorothy; Paul Mary; Napierala Maureen; Hryciuk Jeanne E
来源:Journal of Clinical Microbiology, 2011, 49(6): 2279-2282.
DOI:10.1128/JCM.00261-11

摘要

Expense inherent to molecular diagnostics may prevent laboratories from utilizing real-time PCR for Clostridium difficile infection. Frozen master mix and overnight aliquot modifications of the BD GeneOhm Cdiff assay failed to impact performance indices compared to the package insert protocol ( P >= 0.31), provided accurate results, and decreased reagent expenditure.

  • 出版日期2011-6