A New Model to Study Sleep Deprivation-Induced Seizure

作者：Lucey Brendan P; Leahy Averi; Rosas Regine; Shaw Paul J^*

来源：SLEEP, 2015, 38(5): 777-+.

DOI：10.5665/sleep.4674

• sleep homeostasis
• Drosophila melanogaster
• seizure

Background and Study Objectives: A relationship between sleep and seizures is well-described in both humans and rodent animal models; however, the mechanism underlying this relationship is unknown. Using Drosophila melanogaster mutants with seizure phenotypes, we demonstrate that seizure activity can be modified by sleep deprivation.
Design: Seizure activity was evaluated in an adult bang-sensitive seizure mutant, stress sensitive B (sesB(9ed4)), and in an adult temperature sensitive seizure mutant seizure (sei(ts1)) under baseline and following 12 h of sleep deprivation. The long-term effect of sleep deprivation on young, immature sesB(9ed4) flies was also assessed.
Setting: Laboratory.
Participants: Drosophila melanogaster.
Interventions: Sleep deprivation.
Measurements and Results: Sleep deprivation increased seizure susceptibility in adult sesB(9ed4)/+ and sei(ts1) mutant flies. Sleep deprivation also increased seizure susceptibility when sesB was disrupted using RNAi. The effect of sleep deprivation on seizure activity was reduced when sesB(9ed4)/+ flies were given the anti-seizure drug, valproic acid. In contrast to adult flies, sleep deprivation during early fly development resulted in chronic seizure susceptibility when sesB(9ed4)/+ became adults.
Conclusions: These findings show that Drosophila is a model organism for investigating the relationship between sleep and seizure activity.

• 出版日期2015-5-1