High-Resolution Episcopic Microscopy (HREM): A Tool for Visualizing Skin Biopsies

作者：Geyer Stefan H; Noehammer Maria M; Mathae Markus; Reissig Lukas; Tinhofer Ines E; Weninger Wolfgang J^*

来源：Microscopy and Microanalysis, 2014, 20(5): 1356-1364.

DOI：10.1017/S1431927614013063

• imaging
• 3D
• HREM
• episcopic
• skin

We evaluate the usefulness of digital volume data produced with the high-resolution episcopic microscopy (HREM) method for visualizing the three-dimensional (3D) arrangement of components of human skin, and present protocols designed for processing skin biopsies for HREM data generation. A total of 328 biopsies collected from normally appearing skin and from a melanocytic nevus were processed. Cuboidal data volumes with side lengths of similar to 2 x 3 x 6 mm(3) and voxel sizes of 1.07 x 1.07 x 1.5 mu m(3) were produced. HREM data fit ideally for visualizing the epidermis at large, and for producing highly detailed volume and surface-rendered 3D representations of the dermal and hypodermal components at a structural level. The architecture of the collagen fiber bundles and the spatial distribution of nevus cells can be easily visualized with volume-rendering algorithms. We conclude that HREM has great potential to serve as a routine tool for researching and diagnosing skin pathologies.

• 出版日期2014-10
• 单位河北医科大学