牛血清白蛋白在锁阳RAPD分析中的作用(英文)

作者：周玉碧; 叶润蓉; 卢学峰; 刘洋; 彭敏

来源：Agricultural Science & Technology, 2007, Z1: 64-67.

• BSA
• Cynomorium songaricum
• RAPD

[Objective] The objective of the study was to study the role played by BSA in Random Amplified polymorphic DNA(RAPD).[Method]The improvement of RAPD amplification was observed by adding BSA in the analysis of Cynomorium songaricum RAPD.[Result] The result showed that in the process of Cynomorium songaricum RAPD analysis,the effect of amplification improved significantly and the dosage of Taq enzyme declined by adding BSA.The suitable concentration of BSA used in RAPD of Cynomorium songaricum Rupr was 2 μg/μl.[Conclusion]It is feasible to add BSA to improve the RAPD analysis of plants.This study has provided foundation for the application of BSA in RAPD analysis.

• 出版日期2007-12-15
• 单位中国科学院西北高原生物研究所