Design and Use of Peptide-Based Antibodies Decreasing Superoxide Production by Mitochondrial Complex I and Complex II

作者：Kang Patrick T; Yun June; Kaumaya Pravin P T; Chen Yeong Renn^*

来源：Biopolymers, 2011, 96(2): 207-221.

DOI：10.1002/bip.21457

• peptide
• antibody
• superoxide generation
• complex I
• complex II

Mitochondria are the major source of reactive oxygen species. Both complex I and complex II mediate O-2(center dot-): production in mitochondria and host reactive proteinthiols. To explore the functions of the specific domains involved in the redox modifications of complexes land II, various peptide-based antibodies were generated against these complexes, and their inhibitory effects were subsequently measured. The redox domains involved in S-glutathionylation and nitration, as well as the binding motif of the iron sulfur cluster (N1a) of the complexes I and II were utilized to design B-cell epitopes for generating antibodies. The effect of antibody binding on enzyme-mediated O-2(center dot-): generation was measured by EPR spin trapping. Binding of either antibody AbGSCA206 or AbGSCB367 against glutathione (GS)-binding domain to complex 1 inhibit its O-2(center dot-) generation, but does not affect electron transfer efficiency. Binding of antibody (Ab24N1a) against the binding motif of N1a to complex I modestly suppresses both O-2(center dot-): generation and electron transfer efficiency. Binding of either antibody Ab75 or Ab24 against nonredox domain decreases electron leakage for O-2(center dot-) production. In complex II, binding of antibody AbGSC90 against GS-binding domain to complex II; marginally decreases both O-2(center dot-) generation and electron transfer activity. Binding of antibody AbY142 to complex II against the nitrated domain modestly inhibits electron leakage, but does not affect the electron transfer activity of complex II. In conclusion, mediation of O-2(center dot-) generation by complexes I and II can be regulated by specific redox and nonredox domains.

• 出版日期2011