Anti-inflammatory effects of beta-sitosterol-beta-D-glucoside from Trachelospermum jasminoides (Apocynaceae) in lipopolysaccharide-stimulated RAW 264.7 murine macrophages

作者：Choi Ji Na; Choi Yun Hyeok; Lee Jeong Min; Noh Ill Chan; Park Jae Won; Choi Wahn Soo; Choi Jung Ho

来源：Natural Product Research, 2012, 26(24): 2340-2343.

DOI：10.1080/14786419.2012.654608

• anti-inflammation
• Trachelospermum jasminoides
• beta-sitosterol-beta-D-glucoside
• lipopolysaccharide
• RAW 264.7

Trachelospermum jasminoides (Apocynaceae) has pharmacological effects that include anti-inflammatory, anti-bacterial and anti-viral activities, which have been observed from various studies. Of these pharmacological effects, the anti-inflammatory capacity of compounds from T. jasminoides is not yet known exactly. In this study, we investigated the compound that can be used for the suppression of lipopolysacchaide (LPS) stimulated inflammatory responses in macrophages among the five isolated compounds. beta-sitosterol-beta-D-glucoside (1) was found to reduce nitric oxide (NO) production from LPS-induced RAW 264.7 cells the most. In addition, compound 1 strongly inhibited the interleukin 6 (IL-6) activities of stimulated macrophages. Treatment of RAW 264.7 cells with compound 1 reduced secretion of inflammatory elements including tumour necrosis factor - alpha (TNF-alpha) and interleukin 1 beta (IL-1 beta). Thus, compound 1 may be a useful candidate for the development of new drugs to treat endotoxemia and inflammation accompanied by the overproduction of NO.

• 出版日期2012