A RAPID PROCEDURE FOR THE HUMANIZATION OF MONOCLONAL-ANTIBODIES

作者：SANDHU JS^*

来源：Gene, 1994, 150(2): 409-410.

• ANTIBODY HUMANIZATION
• SITE-DIRECTED MUTAGENESIS
• POLYMERASE CHAIN REACTION
• EFFICIENCY

An efficient and rapid procedure for the humanization of murine monoclonal antibodies (MumAb) is described. It consists of site-directed mutagenesis (SDM) to transfer the murine complementarity-determining regions (MuCDR) onto human framework regions (HuFR), followed by polymerase chain reaction (PCR) of the SDM product. Using SDM/PCR, rapid and correct humanization of MumAb heavy chains is clearly demonstrated. Compared to current protocols this method considerably reduces the time and labour required to generate humanized mAb.

• 出版日期1994-12-15