Size Dependent Cellular Uptake of Rod-like Bionanoparticles with Different Aspect Ratios

作者：Liu, Xiangxiang; Wu, Fengchi; Tian, Ye; Wu, Man; Zhou, Quan; Jiang, Shidong; Niu, Zhongwei^*

来源：Scientific Reports, 2016, 6(1): 24567.

DOI：10.1038/srep24567

• TOBACCO-MOSAIC-VIRUS
• RECEPTOR-MEDIATED ENDOCYTOSIS
• VIRAL NANOPARTICLES
• PARTICLE DESIGN
• NUCLEIC-ACID
• PROTEIN
• SHAPE
• BIODISTRIBUTION
• RECONSTITUTION
• TRAFFICKING

Understanding the cellular internalization mechanism of nanoparticles is essential to study their biological fate. Especially, due to the anisotropic properties, rod-like nanoparticles have attracted growing interest for the enhanced internalization efficiency with respect to spherical nanoparticles. Here, to elucidate the effect of aspect ratio of rod-like nanoparticles on cellular uptake, tobacco mosaic virus (TMV), a typical rod-like bionanoparticle, is developed as a model. Nanorods with different aspect ratios can be obtained by ultrasound treatment and sucrose density gradient centrifugation. By incubating with epithelial and endothelial cells, we found that the rod-like bionanoparticles with various aspect ratios had different internalization pathways in different cell lines: microtubules transport in HeLa and clathrin-mediated uptake in HUVEC for TMV4 and TMV8; caveolae-mediated pathway and microtubules transport in HeLa and HUVEC for TMV17. Differently from most nanoparticles, for all the three TMV nano-rods with different aspect ratios, macropinocytosis takes no effect on the internalization in both cell types. This work provides a fundamental understanding of the influence of aspect ratio on cellular uptake decoupled from charge and material composition.

• 出版日期2016-4-15
• 单位中国科学院理化技术研究所; 中国科学院大学