A simple in chemico method for testing skin sensitizing potential of chemicals using small endogenous molecules

作者：Nepal Mahesh Raj; Shakya Rajina; Kang Mi Jeong; Jeong Tae Cheon^*

来源：Toxicology Letters, 2018, 289: 75-85.

DOI：10.1016/j.toxlet.2018.03.006

• High-performance liquid chromatography
• Cysteamine
• Glutathione
• In chemico
• Derivatization
• Alternative test

Among many of the validated methods for testing skin sensitization, direct peptide reactivity assay (DPRA) employs no cells or animals. Although no immune cells are involved in this assay, it reliably predicts the skin sensitization potential of a chemical in chemico. Herein, a new method was developed using endogenous small-molecular-weight compounds, cysteamine and glutathione, rather than synthetic peptides, to differentiate skin sensitizers from non-sensitizers with an accuracy as high as DPRA. The percent depletion of cysteamine and glutathione by test chemicals was measured by an HPLC equipped with a PDA detector. To detect small-size molecules, such as cysteamine and glutathione, a derivatization by 4-(4-dimethylaminophenylazo) benzenesulfonyl chloride (DABS-Cl) was employed prior to the HPLC analysis. Following test method optimization, a cutoff criterion of 7.14% depletion was applied to differentiate skin sensitizers from non-sensitizers in combination of the ratio of 1: 25 for cysteamine: test chemical with 1: 50 for glutathione: test chemical for the best predictivity among various single or combination conditions. Although overlapping HPLC peaks could not be fully resolved for some test chemicals, high levels of sensitivity (100.0%), specificity (81.8%), and accuracy (93.3%) were obtained for 30 chemicals tested, which were comparable or better than those achieved with DPRA.

• 出版日期2018-6-1