Anticancer activity test of ethyl acetate extract of endophytic fungi isolated from soursop leaf (Annona muricata L.)

作者：Minarni; Artika I Made; Julistiono Heddy; Bermawie Nurliani; Riyanti Eny Ida; Hasim; Hasan Akhmad Endang Zainal^*

来源：Asian Pacific Journal of Tropical Medicine, 2017, 10(6): 635-640.

DOI：10.1016/j.apjtm.2017.06.004

• Annona muricata
• Anticancer MCF 7
• Endophytic fungi
• Soursop leaf

Objective: To analyze anticancer activity of an ethyl acetate extract of endophytic fungi isolated from soursop leaf (Annona muricata L.). Methods: Anticancer activity of fungal extracts was determined by observing its toxicity against MCF-7 (Michigan Cancer Foundation-7) cells in vitro by using the 3(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide (MTT) assay method. At an extract concentration of 100 ughnL, 4 isolates out of 12 showed high activity against the cancer cell growth. The four isolates were then selected for further IC50 determination, by measuring the inhibition of cancer cell proliferation at extract concentration of 25 mu g/mL, 50 pg/mL, 100 mu g/mL. 200 puhnL and 400 mu g/mL. Results: Results showed that isolate Sir-G5 had the highest anticancer activity with an IC, of 19.20 IV mL. The best isolates were screened again using a normal cell (Chang cells) to determine its toxicity against normal cells. Results indicated that the extracts do not affect the proliferation of normal cells. Molecular identification showed that the fungal isolate Sir-G5 has a close relationship with Phonwpsis sp. Conclusions: The endophytic fungi isolated from soursop leaf has the potential to be used as a source of anticancer agents.

• 出版日期2017-6
• 单位河北医科大学