Microchip platforms for multiplex single-cell functional proteomics with applications to immunology and cancer research

作者：Wei Wei; Shin Young Shik; Ma Chao; Wang Jun; Elitas Meltem; Fan Rong; Heath James R^*

来源：Genome Medicine, 2013, 5(8): 75.

DOI：10.1186/gm479

Single-cell functional proteomics assays can connect genomic information to biological function through quantitative and multiplex protein measurements. Tools for single-cell proteomics have developed rapidly over the past 5 years and are providing approaches for directly elucidating phosphoprotein signaling networks in cancer cells or for capturing high-resolution snapshots of immune system function in patients with various disease conditions. We discuss advances in single-cell proteomics platforms, with an emphasis on microchip methods. These methods can provide a direct correlation of morphological, functional and molecular signatures at the single-cell level. We also provide examples of how those platforms are being applied to both fundamental biology and clinical studies, focusing on immune-system monitoring and phosphoprotein signaling networks in cancer.

• 出版日期2013-8-29