A Micro-Flow-Batch Analyzer using Webcam for Spectrophotometric Determination of Ortho-phosphate and Aluminium(III) in Tap Water

作者：Lima Marcelo B; Andrade Stefani I E; Neta Maria S Silva; Barreto Inaka S; Almeida Luciano F; Araujo Mario Cesar U^*

来源：Journal of the Brazilian Chemical Society, 2014, 25(5): 898-906.

DOI：10.5935/0103-5053.20140059

• micro-flow-batch analyzer
• digital images
• RGB color system
• webcam
• tap water

In this work, a micro-flow-batch analyzer (mu FBA) using webcam for spectrophotometric determination of ortho-phosphate and aluminium(III) in tap water is proposed. PO4 (3-)and Al3+ determinations were performed by employing molybdenum blue and quecertin methods, respectively. In order to build linear analytical curves, a mathematical model was used on the basis of the RGB (red, green and blue) values. Working ranges were from 25 to 500 mu g L-1 for both analytes with estimated limits of detection, relative standard deviation (RSD, n = 5) and sampling rate of 0.82 mu g L-1, < 0.8% and 160 h(-1) for PO4 (3-) , and 0.93 mu g L-1, < 1.1% and 160 h(-1) for Al(III). Comparing with the reference methods, no statistically significant differences were observed when applying the paired t-test at a 95% confidence level. Recovery study shows results between 98.1 and 102.8%. The proposed mu FBA presented satisfactory portability, robustness, flexibility, low-cost device and reduced chemicals consumption.

• 出版日期2014-5