科研之友
微信
新浪微博
Facebook
分享链接
摘要
The detection of mercury in biological systems and its imaging is of highly importance. In this work, a ratiometric fluorescence sensor is developed based on fluorescence resonance energy transfer (FRET) with N-acetyl-L-cysteine functionalized quantum dots (NAC-QDs) as donor and Rhodamine 6G derivative-mercury conjugate (R6G-D-Hg) as acceptor. Mercury annihilates the fluorescence of NAC-QDs at 508 nm and meanwhile interacts with R6G derivative to form a fluorescent conjugate giving rise to emission at 554 nm. Resonance energy transfer from NAC-QDs to R6G-D-Hg is triggered by mercury resulting in concentration-dependent variation of fluorescence ratio F-508/F-554. A linear calibration of F-508/F-554 versus mercury concentration is obtained within 5-250 mu g L-1, along with a detection limit of 0.75 mu g L-1 and a RSD of 3.2% (175 mu g L-1). The sensor generates colorimetric images for mercury within 0-250 mu g L-1, facilitating visual detection of mercury with a distinguishing ability of 50 mu g L-1. This feature is further demonstrated by colorimetric imaging of intracellular mercury. On the other hand, the NACQDs/R6G-D FRET sensing system is characterized by a combination of high sensitivity and selectivity. The present study provides an approach for further development of ratiometric sensors dedicated to selective in vitro or in vivo sensing some species of biologically interest.
- 出版日期2013-11-15
- 单位东北大学
